Stanford University Li Ka Shing Conference Center Paul Berg Hall

291 Campus Drive
Stanford, CA 94305

Stanford University Campus Map

Since 2004, Bay Area cryoEM labs have gathered 1-2 times each year for a day-long symposium to share their latest research and discuss up-and-coming methods and technology.

Agenda

8:30 AM - 9:20 AM Check-in/Poster Set-up/Morning Refreshments

9:20 AM - 9:30 AM Welcome Remarks - Prof. Wah Chiu, SLAC National Accelerator Lab/Stranford University

9:30 AM -11:45 AM SINGLE PARTICLE CRYOEM SESSION

• A.1 Single Particle I: Sample Preparation and Data Collection (9:30 AM - 10:30 AM) Chair: Brandon Courteau, UC San Francisco

  • 9:30 AM - 9:50 AM: Matt Braga, UC Davis

    • Investigating the mechanism of GEF switch-mediated enhancement of RhoA activation by PRG using integrative structural biology

  • 9:50 AM - 10:10 AM: Shigeki Nagai, Stanford University

    • Structure of a SWI/SNF-associated transcription pre-initiation complex

  • 10:10 AM - 10:30 AM: Matt Johnson, Genentech

    • CryoShields: Amphipathic Oligopeptides that Accelerate High Throughput Cryo-EM

  • 10:30 AM - 10:45 AM Break

• A.2 Single Particle II: Image Processing, Modeling and Structure Validation (10:45 AM - 11:45 AM) Chair: Jackson Baumgartner, UC Santa Cruz

  • 10:45 AM - 11:05 AM: Allison Maker, UC San Francisco

    • Toward full-length structures of HER family receptors

  • 11:05 AM - 11:25 AM: Michelle Von Merta-Sustarich, San Francisco State University

    • Mechanistic Insight into Allosteric Upregulation of Argininosuccinate Synthetase I (ASS1)

  • 11:25 AM - 11:45 AM: Dari Kimanius, Biohub

    • From CryoET Reconstruction to Molecular Mechanisms: Integrating Structure, Localization, and Sequence Models

• ThermoFisher, Sponsor (11:45 AM - 11:55 AM)

• SubAngstrom, Sponsor (11:55 AM - 12:00 PM)

  • 12:00 PM - 1:30 PM Group Photo and Lunch Break

1:30 PM - 4:45 PM CELLULAR CRYOET AND TISSUE CRYO-VEM SESSION

• B.1 cryoET I: Cell and Tissue Vitrification and Milling (1:30 PM - 2:50 PM) Chair: Jane Lee, Stanford University

  • 1:30 PM - 1:50 PM: Mallak Ali, Biohub

    • Using PP7 Virus-Like Particles (VLPs) in E.Coli as a Benchmark Sample for Cryo-electron Tomography

  • 1:50 PM - 2:10 PM: Phyllis Wang, SLAC National Accelerator Laboratory

    • In situ structural analysis of vesicle-associated NLRP3 driving inflammasome assembly

  • 2:10 PM - 2:30 PM: Yue Yu, Biohub

    • Embrella: Integrated Data Tracking and Processing Workflow Orchestration for Cryo-Electron Tomography

  • 2:30 PM - 2:50 PM: Kevin Rose, Stanford University

    • pH-correlated cryo-ET of lysosomes

  • 2:50 PM - 3:05 PM Break

• B.2 cryo ET II: CryoET and Cryo-vEM (3:05 PM - 4:45 PM) Chair: Joshua Dickerson, UC Berkeley

  • 3:05 PM - 3:25 PM: Julia Peukes, Biohub

    • Fluorescence-guided FIB milling with high accuracy

  • 3:25 PM - 3:45 PM: Xavier Heiligenstein, CryoCapCell

    • Live-HPF-CLEM - Vitrifying an Autogrid to optimize a Cryo-Workflow

  • 3:45 PM - 4:05 PM: Bharti Singal, Stanford University

    • A comprehensive study for multi-scale organoid analysis

  • 4:05 PM - 4:25 PM: Lydia-Marie Joubert, SLAC National Accelerator Laboratory

    • Integration of cryo-ET with cryo-Volume EM: a new frontier to explore

  • 4:25 PM - 4:45 PM: Arthur Melo, UC San Francisco

    • Using SEM Imaging to Understand Neurodegeneration Across Scales: Navigating Resolution, Throughput, and Complexity

4:45 PM - 6:45 PM: Poster Session and Reception

Campus Parking Logistics:

Meeting Contacts:

Program Contact: Jane Lee

Administrative Contact: JoAnn Polizzi

Sponsored by: